EZ Nucleosomal DNA Prep KitThe EZ Nucleosomal DNA Prep Kit is a streamlined procedure for the isolation of nucleosome associated DNA. The kit includes reagents procedures for the following: cell nuclei isolation, intact nuclei enzymatic digestion, and nucleosomal DNA purification. This kit includes two different enzymes for nucleosomal DNA preparation: Atlantis dsDNase and Micrococcal Nuclease. Enzymatic digestion yields very homogeneous populations of core nucleosomes and

as well as enzyme-digested and mechanically sheared fragments

Buffer neutralization and lysate separation steps are expedited using a specially designed Neutralization Buffer

is then used to isolate the DNA

primers) from double stranded species (e

a unique single-step RNA extraction/binding buffer

This enzyme is compatible with low volume inputs

Opaque black in color

Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA > 50 kb to be eluted in as little as 10 µl

Since the methylated DNA (pUC19m) contains a Nco I restriction site (the non-methylated (pUC19) DNA does not)

RT-PCR-ready RNA

RNA Extraction Buffer is used for RNA isolation in the Pinpoint Slide RNA Isolation Systems I & II

DNA is eluted with a small volume of water or loading dye containing formamide